Epithelial-to-Mesenchymal Transition Enhances Cancer Cell Sensitivity to Cytotoxic Effects of Cold Atmospheric Plasmas in Breast and Bladder Cancer Systems

Cold atmospheric plasma (CAP) has emerged as a highly selective anticancer agent, most recently in the form of plasma-activated medium (PAM). Since epithelial–mesenchymal transition (EMT) been implicated resistance to various cancer therapies, we assessed whether EMT status is associated with PAM response. Mesenchymal breast cell lines, well mesenchymal variant an isogenic EMT/MET human system (PMC42-ET/LA), were more sensitive treatment than their epithelial counterparts, contrary responses other therapies. The same trend was seen luminal muscle-invasive bladder model (TSU-Pr1/B1/B2) and non-muscle-invasive basal 5637 line. Three-dimensional spheroid cultures lines less compared two-dimensional counterparts; however, incrementally better again mesenchymally-shifted lines. This study provides evidence that preferentially inhibits carcinoma cells, which have Thus, may represent novel can selectively inhibit triple-negative cancers subset aggressive cancers, tend be mesenchymal. Our approach potentially utilized for exhibiting opens new opportunities CAP promising onco-therapy.